collagen 2 (SouthernBiotech)
Structured Review

Collagen 2, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 93/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goat+anti+collagen/Goat+Anti-Type+II+Collagen-UNLB/pmc12971017-76-5-12
Average 93 stars, based on 30 article reviews
Images
1) Product Images from "β 2 -adrenoceptor signaling in chondrocytes regulates long bone growth, bone homeostasis, and fracture healing in mice"
Article Title: β 2 -adrenoceptor signaling in chondrocytes regulates long bone growth, bone homeostasis, and fracture healing in mice
Journal: JBMR Plus
doi: 10.1093/jbmrpl/ziag021
Figure Legend Snippet: Long bone growth of 12-wk-old male Adrb2 flox/flox and Adrb2 Col2a1-Cre mice. (A) Femur and (B) tibia length. (C) Femoral growth plate thickness. Thickness of the (D) non-hypertrophic and (E) hypertrophic zone of the femoral growth plate. (F) Collagen 10 + chondrocytes, (G) collagen 2 + chondrocytes and (H) runt-related transcription factor 2 (Runx2) + chondrocytes in the femoral growth plate. (I) Histological (toluidine blue) and immunohistochemical (collagen 10, collagen 2, Runx2) staining of the femoral growth plate. Scale bar = 50 μ m . n = 4-6. * p < .05, ** p < .01, **** p < .0001.
Techniques Used: Immunohistochemical staining, Staining
Figure Legend Snippet: Long bone growth of 12-wk-old female Adrb2 flox/flox and Adrb2 Col2a1-Cre mice. (A) Femur and (B) tibia length. (C) Femoral growth plate thickness. Thickness of the (D) non-hypertrophic and (E) hypertrophic zone of the femoral growth plate. (F) Collagen 10 + chondrocytes, (G) collagen 2 + chondrocytes, and (H) runt-related transcription factor 2 (Runx2) + chondrocytes in the femoral growth plate. (I) Histological (toluidine blue) and immunohistochemical (collagen 10, collagen 2, Runx2) staining of the femoral growth plate. Scale bar = 50 μ m . n = 4-6. * p < .05.
Techniques Used: Immunohistochemical staining, Staining
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Following a 1 h incubation in blocking solution (PBS + 0.2% bovine serum albumin (BSA)), all the slides were incubated with the following primary antibodies overnight at 4 °C: Article Title: Molecular imaging in experimental pulmonary fibrosis reveals that nintedanib unexpectedly modulates CCR2 immune cell infiltration Article Snippet: Background Pulmonary fibrosis is a challenging clinical problem with lung pathology featuring immune cell infiltrates, fibroblast expansion, and matrix deposition.. Molecular analysis of diseased lungs and preclinical models have uncovered C–C chemokine receptor type 2 (CCR2)+ monocyte egress from the bone marrow into the lung, where they acquire profibrotic activities.. Current drug treatment is focused on fibroblast activity. Article Title: Biochemical and Histological Differences between Longitudinal and Vertical Fibres of Dupuytren's Palmar Aponeurosis and Innovative Clinical Implications. Article Snippet: The slides for Collagen I were treated using an incubation of EDTA (Ethylenediaminetetraacetate—Sigma Aldrich, St. Louis, MO, USA) pH 9, at 95 ◦C for 15 min, to permit the antigen retrieval, and were then washed in PBS. .. Following a 1 h incubation in blocking solution (PBS + 0.2% bovine serum albumin (BSA)), all the slides were incubated with the following primary antibodies overnight at 4 ◦C: Blocking Assay:Article Title: Biochemical and Histological Differences between Longitudinal and Vertical Fibres of Dupuytren's Palmar Aponeurosis and Innovative Clinical Implications. Article Snippet: The slides for Collagen I were treated using an incubation of EDTA (Ethylenediaminetetraacetate—Sigma Aldrich, St. Louis, MO, USA) pH 9, at 95 °C for 15 min, to permit the antigen retrieval, and were then washed in PBS. .. Following a 1 h incubation in blocking solution (PBS + 0.2% bovine serum albumin (BSA)), all the slides were incubated with the following primary antibodies overnight at 4 °C: Article Title: Strategy for drug repurposing in fibroadipogenic replacement during muscle wasting: application to duchenne muscular dystrophy. Article Snippet: .. Plates were rinsed with PBS and fixed with 4% PFA for 10 min. After washing steps and blocking with casein (ThermoFisher) solution for 30 min, Article Title: Strategy for drug repurposing in fibroadipogenic replacement during muscle wasting: application to duchenne muscular dystrophy Article Snippet: .. Plates were rinsed with PBS and fixed with 4% PFA for 10 min. After washing steps and blocking with casein (ThermoFisher) solution for 30 min, Article Title: Biochemical and Histological Differences between Longitudinal and Vertical Fibres of Dupuytren's Palmar Aponeurosis and Innovative Clinical Implications. Article Snippet: The slides for Collagen I were treated using an incubation of EDTA (Ethylenediaminetetraacetate—Sigma Aldrich, St. Louis, MO, USA) pH 9, at 95 ◦C for 15 min, to permit the antigen retrieval, and were then washed in PBS. .. Following a 1 h incubation in blocking solution (PBS + 0.2% bovine serum albumin (BSA)), all the slides were incubated with the following primary antibodies overnight at 4 ◦C: Activity Assay:Article Title: Molecular imaging in experimental pulmonary fibrosis reveals that nintedanib unexpectedly modulates CCR2 immune cell infiltration Article Snippet: Background Pulmonary fibrosis is a challenging clinical problem with lung pathology featuring immune cell infiltrates, fibroblast expansion, and matrix deposition.. Molecular analysis of diseased lungs and preclinical models have uncovered C–C chemokine receptor type 2 (CCR2)+ monocyte egress from the bone marrow into the lung, where they acquire profibrotic activities.. Current drug treatment is focused on fibroblast activity. Binding Assay:Article Title: Molecular imaging in experimental pulmonary fibrosis reveals that nintedanib unexpectedly modulates CCR2 immune cell infiltration Article Snippet: Background Pulmonary fibrosis is a challenging clinical problem with lung pathology featuring immune cell infiltrates, fibroblast expansion, and matrix deposition.. Molecular analysis of diseased lungs and preclinical models have uncovered C–C chemokine receptor type 2 (CCR2)+ monocyte egress from the bone marrow into the lung, where they acquire profibrotic activities.. Current drug treatment is focused on fibroblast activity. |