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collagen 2  (SouthernBiotech)


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    Structured Review

    SouthernBiotech collagen 2
    Long bone growth of 12-wk-old male Adrb2 flox/flox and Adrb2 Col2a1-Cre mice. (A) Femur and (B) tibia length. (C) Femoral growth plate thickness. Thickness of the (D) non-hypertrophic and (E) hypertrophic zone of the femoral growth plate. (F) Collagen 10 + chondrocytes, (G) <t>collagen</t> <t>2</t> + chondrocytes and (H) runt-related transcription factor 2 (Runx2) + chondrocytes in the femoral growth plate. (I) Histological (toluidine blue) and immunohistochemical (collagen 10, collagen 2, Runx2) staining of the femoral growth plate. Scale bar = 50 μ m . n = 4-6. * p < .05, ** p < .01, **** p < .0001.
    Collagen 2, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 93/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+anti+collagen/Goat+Anti-Type+II+Collagen-UNLB/pmc12971017-76-5-12
    Average 93 stars, based on 30 article reviews
    collagen 2 - by Bioz Stars, 2026-10
    93/100 stars

    Images

    1) Product Images from "β 2 -adrenoceptor signaling in chondrocytes regulates long bone growth, bone homeostasis, and fracture healing in mice"

    Article Title: β 2 -adrenoceptor signaling in chondrocytes regulates long bone growth, bone homeostasis, and fracture healing in mice

    Journal: JBMR Plus

    doi: 10.1093/jbmrpl/ziag021

    Long bone growth of 12-wk-old male Adrb2 flox/flox and Adrb2 Col2a1-Cre mice. (A) Femur and (B) tibia length. (C) Femoral growth plate thickness. Thickness of the (D) non-hypertrophic and (E) hypertrophic zone of the femoral growth plate. (F) Collagen 10 + chondrocytes, (G) collagen 2 + chondrocytes and (H) runt-related transcription factor 2 (Runx2) + chondrocytes in the femoral growth plate. (I) Histological (toluidine blue) and immunohistochemical (collagen 10, collagen 2, Runx2) staining of the femoral growth plate. Scale bar = 50 μ m . n = 4-6. * p < .05, ** p < .01, **** p < .0001.
    Figure Legend Snippet: Long bone growth of 12-wk-old male Adrb2 flox/flox and Adrb2 Col2a1-Cre mice. (A) Femur and (B) tibia length. (C) Femoral growth plate thickness. Thickness of the (D) non-hypertrophic and (E) hypertrophic zone of the femoral growth plate. (F) Collagen 10 + chondrocytes, (G) collagen 2 + chondrocytes and (H) runt-related transcription factor 2 (Runx2) + chondrocytes in the femoral growth plate. (I) Histological (toluidine blue) and immunohistochemical (collagen 10, collagen 2, Runx2) staining of the femoral growth plate. Scale bar = 50 μ m . n = 4-6. * p < .05, ** p < .01, **** p < .0001.

    Techniques Used: Immunohistochemical staining, Staining

    Long bone growth of 12-wk-old female Adrb2 flox/flox and Adrb2 Col2a1-Cre mice. (A) Femur and (B) tibia length. (C) Femoral growth plate thickness. Thickness of the (D) non-hypertrophic and (E) hypertrophic zone of the femoral growth plate. (F) Collagen 10 + chondrocytes, (G) collagen 2 + chondrocytes, and (H) runt-related transcription factor 2 (Runx2) + chondrocytes in the femoral growth plate. (I) Histological (toluidine blue) and immunohistochemical (collagen 10, collagen 2, Runx2) staining of the femoral growth plate. Scale bar = 50 μ m . n = 4-6. * p < .05.
    Figure Legend Snippet: Long bone growth of 12-wk-old female Adrb2 flox/flox and Adrb2 Col2a1-Cre mice. (A) Femur and (B) tibia length. (C) Femoral growth plate thickness. Thickness of the (D) non-hypertrophic and (E) hypertrophic zone of the femoral growth plate. (F) Collagen 10 + chondrocytes, (G) collagen 2 + chondrocytes, and (H) runt-related transcription factor 2 (Runx2) + chondrocytes in the femoral growth plate. (I) Histological (toluidine blue) and immunohistochemical (collagen 10, collagen 2, Runx2) staining of the femoral growth plate. Scale bar = 50 μ m . n = 4-6. * p < .05.

    Techniques Used: Immunohistochemical staining, Staining

    Related Articles

    other:

    Article Title: Molecular imaging in experimental pulmonary fibrosis reveals that nintedanib unexpectedly modulates CCR2 immune cell infiltration
    Article Snippet: For immunostaining, antigen retrieval was performed by heating tissue sections in citrate buffer, pH 6.0 (Antigen Unmasking solution, Vector Laboratories, #H-3300) in a pressure cooker (Biocare Medical, #DC0503) for 3 min followed by 30 min for cooling.

    Incubation:

    Article Title: Biochemical and Histological Differences between Longitudinal and Vertical Fibres of Dupuytren's Palmar Aponeurosis and Innovative Clinical Implications.
    Article Snippet: The slides for Collagen I were treated using an incubation of EDTA (Ethylenediaminetetraacetate—Sigma Aldrich, St. Louis, MO, USA) pH 9, at 95 °C for 15 min, to permit the antigen retrieval, and were then washed in PBS. .. Following a 1 h incubation in blocking solution (PBS + 0.2% bovine serum albumin (BSA)), all the slides were incubated with the following primary antibodies overnight at 4 °C: Goat Anti-Collagen I (Southern-Biotech (Birmingham, AL, USA), 1:400); Mouse Anti-Collagen III (Abcam (Cambridge, UK), 1:300); Mouse Anti-Human Muscle Actin (Clone 1A4-Agilent Dako (Santa Clara, CA, USA), 1:200); Rabbit Anti-Human Von Willebrand Factor (vWF-Agilent Dako, 1:600); Monoclonal Mouse anti-D2-40 (1:100), Monoclonal Mouse Anti-human CD68 (Dako-Agilent, 1:3000). .. After repetitive PBS washing, the sections were incubated with the secondary antibodies conjugated with HRP (Horseradish Peroxidase) for 1 h in PBS + 0.2% BSA/Rabbit Anti-Goat (Jackson ImmunoResearch (West Grove, PA, USA), 1:300); Goat Anti-Mouse (Jackson ImmunoResearch, 1:500); Goat Anti-Rabbit (Jackson ImmunoResearch, 1:250).

    Article Title: Molecular imaging in experimental pulmonary fibrosis reveals that nintedanib unexpectedly modulates CCR2 immune cell infiltration
    Article Snippet: Background Pulmonary fibrosis is a challenging clinical problem with lung pathology featuring immune cell infiltrates, fibroblast expansion, and matrix deposition.. Molecular analysis of diseased lungs and preclinical models have uncovered C–C chemokine receptor type 2 (CCR2)+ monocyte egress from the bone marrow into the lung, where they acquire profibrotic activities.. Current drug treatment is focused on fibroblast activity.

    Article Title: Biochemical and Histological Differences between Longitudinal and Vertical Fibres of Dupuytren's Palmar Aponeurosis and Innovative Clinical Implications.
    Article Snippet: The slides for Collagen I were treated using an incubation of EDTA (Ethylenediaminetetraacetate—Sigma Aldrich, St. Louis, MO, USA) pH 9, at 95 ◦C for 15 min, to permit the antigen retrieval, and were then washed in PBS. .. Following a 1 h incubation in blocking solution (PBS + 0.2% bovine serum albumin (BSA)), all the slides were incubated with the following primary antibodies overnight at 4 ◦C: Goat Anti-Collagen I (Southern-Biotech (Birmingham, AL, USA), 1:400); Mouse Anti-Collagen III (Abcam (Cambridge, UK), 1:300); Mouse Anti-Human Muscle Actin (Clone 1A4-Agilent Dako (Santa Clara, CA, USA), 1:200); Rabbit Anti-Human Von Willebrand Factor (vWF-Agilent Dako, 1:600); Monoclonal Mouse anti-D2-40 (1:100), Monoclonal Mouse Anti-human CD68 (Dako-Agilent, 1:3000). .. After repetitive PBS washing, the sections were incubated with the secondary antibodies conjugated with HRP (Horseradish Peroxidase) for 1 h in PBS + 0.2% BSA/Rabbit AntiGoat (Jackson ImmunoResearch (West Grove, PA, USA), 1:300); Goat Anti-Mouse (Jackson ImmunoResearch, 1:500); Goat Anti-Rabbit (Jackson ImmunoResearch, 1:250).

    Blocking Assay:

    Article Title: Biochemical and Histological Differences between Longitudinal and Vertical Fibres of Dupuytren's Palmar Aponeurosis and Innovative Clinical Implications.
    Article Snippet: The slides for Collagen I were treated using an incubation of EDTA (Ethylenediaminetetraacetate—Sigma Aldrich, St. Louis, MO, USA) pH 9, at 95 °C for 15 min, to permit the antigen retrieval, and were then washed in PBS. .. Following a 1 h incubation in blocking solution (PBS + 0.2% bovine serum albumin (BSA)), all the slides were incubated with the following primary antibodies overnight at 4 °C: Goat Anti-Collagen I (Southern-Biotech (Birmingham, AL, USA), 1:400); Mouse Anti-Collagen III (Abcam (Cambridge, UK), 1:300); Mouse Anti-Human Muscle Actin (Clone 1A4-Agilent Dako (Santa Clara, CA, USA), 1:200); Rabbit Anti-Human Von Willebrand Factor (vWF-Agilent Dako, 1:600); Monoclonal Mouse anti-D2-40 (1:100), Monoclonal Mouse Anti-human CD68 (Dako-Agilent, 1:3000). .. After repetitive PBS washing, the sections were incubated with the secondary antibodies conjugated with HRP (Horseradish Peroxidase) for 1 h in PBS + 0.2% BSA/Rabbit Anti-Goat (Jackson ImmunoResearch (West Grove, PA, USA), 1:300); Goat Anti-Mouse (Jackson ImmunoResearch, 1:500); Goat Anti-Rabbit (Jackson ImmunoResearch, 1:250).

    Article Title: Strategy for drug repurposing in fibroadipogenic replacement during muscle wasting: application to duchenne muscular dystrophy.
    Article Snippet: .. Plates were rinsed with PBS and fixed with 4% PFA for 10 min. After washing steps and blocking with casein (ThermoFisher) solution for 30 min, goat anti-collagen I (1:500; Cat. 1310-01; Southern Biotech, Birmingham, AL) or rabbit anti-perilipin A/B (Cat. P1873–200UL; 1:500; Sigma-Aldrich, St. Louis, MO) was added overnight at 4C. .. After the washing steps, wells were incubated with either donkey anti-goat IRDye800 or donkey anti-mouse IRDye800 secondary antibody (1:1000; Li- COR, Lincoln, NE) and CellTag IRDye 700 (Li-COR) for 1 h. After washing with PBS, the fluorescent signal was measured by an Odyssey Imaging system (Li-COR) and normalized by cell number measured in the 700 nm channel.

    Article Title: Strategy for drug repurposing in fibroadipogenic replacement during muscle wasting: application to duchenne muscular dystrophy
    Article Snippet: .. Plates were rinsed with PBS and fixed with 4% PFA for 10 min. After washing steps and blocking with casein (ThermoFisher) solution for 30 min, goat anti-collagen I (1:500; Cat. 1310-01; Southern Biotech, Birmingham, AL) or rabbit anti-perilipin A/B (Cat. P1873–200UL; 1:500; Sigma-Aldrich, St. Louis, MO) was added overnight at 4C. .. After the washing steps, wells were incubated with either donkey anti-goat IRDye800 or donkey anti-mouse IRDye800 secondary antibody (1:1000; Li- COR, Lincoln, NE) and CellTag IRDye 700 (Li-COR) for 1 h. After washing with PBS, the fluorescent signal was measured by an Odyssey Imaging system (Li-COR) and normalized by cell number measured in the 700 nm channel.

    Article Title: Biochemical and Histological Differences between Longitudinal and Vertical Fibres of Dupuytren's Palmar Aponeurosis and Innovative Clinical Implications.
    Article Snippet: The slides for Collagen I were treated using an incubation of EDTA (Ethylenediaminetetraacetate—Sigma Aldrich, St. Louis, MO, USA) pH 9, at 95 ◦C for 15 min, to permit the antigen retrieval, and were then washed in PBS. .. Following a 1 h incubation in blocking solution (PBS + 0.2% bovine serum albumin (BSA)), all the slides were incubated with the following primary antibodies overnight at 4 ◦C: Goat Anti-Collagen I (Southern-Biotech (Birmingham, AL, USA), 1:400); Mouse Anti-Collagen III (Abcam (Cambridge, UK), 1:300); Mouse Anti-Human Muscle Actin (Clone 1A4-Agilent Dako (Santa Clara, CA, USA), 1:200); Rabbit Anti-Human Von Willebrand Factor (vWF-Agilent Dako, 1:600); Monoclonal Mouse anti-D2-40 (1:100), Monoclonal Mouse Anti-human CD68 (Dako-Agilent, 1:3000). .. After repetitive PBS washing, the sections were incubated with the secondary antibodies conjugated with HRP (Horseradish Peroxidase) for 1 h in PBS + 0.2% BSA/Rabbit AntiGoat (Jackson ImmunoResearch (West Grove, PA, USA), 1:300); Goat Anti-Mouse (Jackson ImmunoResearch, 1:500); Goat Anti-Rabbit (Jackson ImmunoResearch, 1:250).

    Activity Assay:

    Article Title: Molecular imaging in experimental pulmonary fibrosis reveals that nintedanib unexpectedly modulates CCR2 immune cell infiltration
    Article Snippet: Background Pulmonary fibrosis is a challenging clinical problem with lung pathology featuring immune cell infiltrates, fibroblast expansion, and matrix deposition.. Molecular analysis of diseased lungs and preclinical models have uncovered C–C chemokine receptor type 2 (CCR2)+ monocyte egress from the bone marrow into the lung, where they acquire profibrotic activities.. Current drug treatment is focused on fibroblast activity.

    Binding Assay:

    Article Title: Molecular imaging in experimental pulmonary fibrosis reveals that nintedanib unexpectedly modulates CCR2 immune cell infiltration
    Article Snippet: Background Pulmonary fibrosis is a challenging clinical problem with lung pathology featuring immune cell infiltrates, fibroblast expansion, and matrix deposition.. Molecular analysis of diseased lungs and preclinical models have uncovered C–C chemokine receptor type 2 (CCR2)+ monocyte egress from the bone marrow into the lung, where they acquire profibrotic activities.. Current drug treatment is focused on fibroblast activity.



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    Image Search Results


    Long bone growth of 12-wk-old male Adrb2 flox/flox and Adrb2 Col2a1-Cre mice. (A) Femur and (B) tibia length. (C) Femoral growth plate thickness. Thickness of the (D) non-hypertrophic and (E) hypertrophic zone of the femoral growth plate. (F) Collagen 10 + chondrocytes, (G) collagen 2 + chondrocytes and (H) runt-related transcription factor 2 (Runx2) + chondrocytes in the femoral growth plate. (I) Histological (toluidine blue) and immunohistochemical (collagen 10, collagen 2, Runx2) staining of the femoral growth plate. Scale bar = 50 μ m . n = 4-6. * p < .05, ** p < .01, **** p < .0001.

    Journal: JBMR Plus

    Article Title: β 2 -adrenoceptor signaling in chondrocytes regulates long bone growth, bone homeostasis, and fracture healing in mice

    doi: 10.1093/jbmrpl/ziag021

    Figure Lengend Snippet: Long bone growth of 12-wk-old male Adrb2 flox/flox and Adrb2 Col2a1-Cre mice. (A) Femur and (B) tibia length. (C) Femoral growth plate thickness. Thickness of the (D) non-hypertrophic and (E) hypertrophic zone of the femoral growth plate. (F) Collagen 10 + chondrocytes, (G) collagen 2 + chondrocytes and (H) runt-related transcription factor 2 (Runx2) + chondrocytes in the femoral growth plate. (I) Histological (toluidine blue) and immunohistochemical (collagen 10, collagen 2, Runx2) staining of the femoral growth plate. Scale bar = 50 μ m . n = 4-6. * p < .05, ** p < .01, **** p < .0001.

    Article Snippet: For immunofluorescence double staining of Collagen 2 (goat anti-mouse Col2, 1:50, #1320-01, Southern Biotech) and β 2 -AR (rabbit anti-mouse β 2 -AR, 1:50, #PA5-77283, Thermo Fisher Scientific), decalcified sections of intact and fractured femora were used.

    Techniques: Immunohistochemical staining, Staining

    Long bone growth of 12-wk-old female Adrb2 flox/flox and Adrb2 Col2a1-Cre mice. (A) Femur and (B) tibia length. (C) Femoral growth plate thickness. Thickness of the (D) non-hypertrophic and (E) hypertrophic zone of the femoral growth plate. (F) Collagen 10 + chondrocytes, (G) collagen 2 + chondrocytes, and (H) runt-related transcription factor 2 (Runx2) + chondrocytes in the femoral growth plate. (I) Histological (toluidine blue) and immunohistochemical (collagen 10, collagen 2, Runx2) staining of the femoral growth plate. Scale bar = 50 μ m . n = 4-6. * p < .05.

    Journal: JBMR Plus

    Article Title: β 2 -adrenoceptor signaling in chondrocytes regulates long bone growth, bone homeostasis, and fracture healing in mice

    doi: 10.1093/jbmrpl/ziag021

    Figure Lengend Snippet: Long bone growth of 12-wk-old female Adrb2 flox/flox and Adrb2 Col2a1-Cre mice. (A) Femur and (B) tibia length. (C) Femoral growth plate thickness. Thickness of the (D) non-hypertrophic and (E) hypertrophic zone of the femoral growth plate. (F) Collagen 10 + chondrocytes, (G) collagen 2 + chondrocytes, and (H) runt-related transcription factor 2 (Runx2) + chondrocytes in the femoral growth plate. (I) Histological (toluidine blue) and immunohistochemical (collagen 10, collagen 2, Runx2) staining of the femoral growth plate. Scale bar = 50 μ m . n = 4-6. * p < .05.

    Article Snippet: For immunofluorescence double staining of Collagen 2 (goat anti-mouse Col2, 1:50, #1320-01, Southern Biotech) and β 2 -AR (rabbit anti-mouse β 2 -AR, 1:50, #PA5-77283, Thermo Fisher Scientific), decalcified sections of intact and fractured femora were used.

    Techniques: Immunohistochemical staining, Staining